General Cell Based Assays
Cell Biolabs Inc Cellular Senescence Detection Kit (SA-Beta-Gal Staining), 50 assays
Senescent cells show common biochemical markers such as expression of an acidic senescence-associated ß-galactosidase (SA-ß-Gal) activity. While senescence has been characterized primarily in cultured cells, there is also evidence that it occurs in vivo. Cells expressing markers of senescence such as SA-ß-Gal have been identified in normal tissues. Cell Biolabs' Cellular Senescence Assay Kit provides an easy-to-use and efficient method to determine cellular senescence. SA-β-galactosidase catalyzes the hydrolysis of X-gal, which produces a blue color. Each kit provides sufficient quantities to perform up to 50 assays in 35 mm wells.
Eurofins Discoverx PathHunter eXpress SREBP2 Nuclear Translocation Assay, 2pl Kit. (93-0830E3CP5M)
The PathHunter eXpress SREBP2 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the cholesterol biosynthesis pathway. This kit assesses ligand (e.g. 5'-UMP) based pathway activation via detection of resulting SREBP2 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged SREBP2 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of SREBP2 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGER4 HEK 293 β-Arrestin GPCR Assay, 10pl Kit. (93-0304E1CP0L)
The PathHunter PTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER4 and EA-tagged β-Arrestin-2. Activation of PTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR25 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0368E2ACP2L)
The PathHunter GPR25 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR25 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR25 and EA-tagged β-Arrestin-2. Activation of GPR25 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR4 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0369E2ACP1L)
The PathHunter GPR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR4 and EA-tagged β-Arrestin-2. Activation of GPR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress INSRb Functional Assay, 2-Plate Kit. (93-0466E3CP16M)
The PathHunter eXpress INSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated INSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPY1R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0397E2CP0M)
The PathHunter NPY1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. peptide YY) based activation of NPY1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPY1R and EA-tagged β-Arrestin-2. Activation of NPY1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TrkB Functional Assay, 2-Plate Kit. (93-0463E3CP16M)
The PathHunter eXpress TrkB Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR37L1 CHO-K1 β-Arrestin Orphan GPCR Assay, 10pl Kit. (93-0378E2ACP1L)
The PathHunter GPR37L1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR37L1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR37L1 and EA-tagged β-Arrestin-2. Activation of GPR37L1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Lonza Walkersville PyroGene Bulk Recombinant Factor C Endpoint Fluorescent Assay
The PyroGene Recombinant Factor C Assay is the evolution of endotoxin detection testing. Lonza scientists have developed a recombinant form of Factor C, the first component in the horseshoe crab clotting cascade activated by endotoxin.Recombinant Factor C(rFC) is activated by endotoxin binding, and the active enzyme then cleaves a synthetic substrate, resulting in the generation of a fluorogenic compound.PyroGene rFC works through a single enzymatic step compared to the multiple step enzymatic process necessary for LAL assays.Benefits:Sensitivity range from 0.005 to 5 EU/mlHigher endotoxin specificity through the elimination of false positive glucan reactionsLess lot-to-lot variabilitySecurity of supplyFDA acknowledged alternative to LALApplications:In-process testingFinal product release testingWater testingTesting plant-based materialContent:30 x 96 tests/vialrFC enzyme solution30 x 6 mL/vial fluorogenic substrate30 x 5 mL/vial rFC assay buffer10 vials endotoxin
Cell Signaling Technology Mast Cell ChymaseCMA1 F6S4Q Rabbit mAb 20 ul
Mast Cell ChymaseCMA1 F6S4Q Rabbit mAb 20 ul
Enzo Life Sciences MMP-1 Assay Buffer (20ml)
Formulation: Liquid. In 50mM HEPES, pH 7.5, 10mM CaCl2 and 0.05% Brij-35. Long Term Storage: Ambient.
Eurofins Discoverx PathHunter Flash Detection Kit, 125 (10 plates), 1,000/4,000 dp (96-well/384-well). (93-0247)
The PathHunter Flash Detection Kit is a detection kit intended for use with PathHunter cell lines or with custom cell lines made using ProLabel/PK expression vectors. This kit will provide a brighter signal with shorter kinetics than the PathHunter Detection Kit, at the cost of a shorter half life. It can be substituted in and used with any cell line that specifies use of the PathHunter Detection kit. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 10 plates (96/384-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0247.
American Research Products Inc Porcine uPA ELISA Kit
This Porcine Plasminogen Activator Urokinase (uPA) ELISA Kit is a precoated Sandwich ELISA assay designed to detect and quantify the target analyte in serum plasma and other biological fluids This product is for Research Use Only