General Cell Based Assays
Abcam Apicidin, HDAC inhibitor, 1MG
MW 623.8 Da, Purity >95%. Potent histone deacetylase inhibitor (IC₅₀ = 0.7 nM). Inhibits cancer cell proliferation via induction of p21WAF1/Cip1 and gelsolin. Active in vitro and in vivo. Cell-permeable.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Lovastatin (Mevinolin), HMG-CoA reductase inhibitor, 10MG
MW 404.5 Da, Purity >98%. Potent, competitive HMG-CoA reductase inhibitor (Ki = 0.6 nM). Blood-brain barrier permeable. Inhibits in vivo and in vitro cholesterol biosynthesis. Also reduces α-synuclein levels in vitro and in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc DAPRed – Autophagy Detection (5 nmol)
DAPRed is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. The small fluorescent molecule DAPRed is used to detect autophagosomes and autolysosomes. The mechanism suggests that the dye is incorporated into the autophagosome during double-membrane formation via structural features and then emits fluorescence under hydrophobic conditions. DALRed is cell-permeable, has no requirement of transfection method, and enables live-cell imaging with fluorescence microscopy. Excitation and emission of DAPRed: λex: 530 nm, λem: 720 nm. For monitoring only autolysosomes, we recommend DALGreen [D675] as it enables the detection of phagosome-lysosome fusion. Dojindo offers various reagents for autophagy research. (https://www.dojindo.com/autophagy-research/#jmp-b)
American Research Products Inc D SORBITOL KIT COLORIMETRIC BA
5000265868 D SORBITOL KIT COLORIMETRIC BA
Cayman Chemical Estradiol 3-(β-D-Glucuronide) (sodium salt), Purity: ≥95%, CAS: 14982-12-8, 10 mg
A non-cholestatic regioisomer of E217G that acts as a substrate for MRP2, competing with E217G for MRP2-mediated transport (IC50 = 14.2 µM)
Cayman Chemical Estradiol 17-(β-D-Glucuronide) (sodium salt hydrate), Purity: ≥95%, 5 mg
An estrogen metabolite that acts as a substrate of MRP2 (Km = 75 µM), and through MRP2-mediated transport, functions as a cholestatic agent, decreasing bile flow
Cayman Chemical (Glp1)-Apelin-13 (trifluoroacetate salt), Purity: ≥95%, 1 mg
A pyroglutamylated form of apelin-13, in that the N-terminal glutamine residue is cyclized to pyroglutamic acid; a potent APJ receptor agonist (EC50 = 0.30 nM); the major apelin isoform in the plasma of healthy human subjects
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-Rb (Ser807/811) Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-Rb (Ser807/811) Detection Kit, 500 Assay Points
Bioassay Systems QuantiChrom™ Boron Assay Kit. Quantitative determination of boron in agricultural and environmental samples.
For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.
Abcam Neuraminidase Assay Kit (Fluorometric - Blue)
Neuraminidase Assay Kit (Fluorometric - Blue)
The product is subject to the following: Abcam Restricted Use Statement
Indigo Biosciences INDIGO Biosciences Reporter Assay System, Human ADRB3, 96-well, Cell-based
This 96-well Human Adrenoceptor Beta 3 (ADRB3) Reporter Assay System screens test compounds by utilizing proprietary human cells. These cells constitutively express Human ADRB3, featuring a luciferase reporter gene linked to cyclic AMP Response Elements. The system quantifies luciferase activity to measure drug-induced ADRB3 changes, allowing assessment of activating or inhibitory functional activities.
- Utilizes human cells engineered for ADRB3 expression.
- Measures ADRB3 activity via a luciferase reporter gene linked to CRE.
- Cryo-preserved reporter cells for immediate dispensing.
- All-inclusive kit contains cells, media, activator, detection reagents, and assay plate.
- Offers high sensitivity with broad linear dynamic range.
- Provides stable light emission for 5 to 90+ minutes.
- Facilitates antagonist-mode assay setup with Isoproterenol.